Journal: Neuroscience Insights

Article Title: Cerebral Proteomic Changes in the rTg-D Rat Model of Cerebral Amyloid Angiopathy Type-2 With Cortical Microhemorrhages and Cognitive Impairments

doi: 10.1177/26331055241288172

Figure Lengend Snippet: Arteriolar deposition of fibrillar amyloid and loss of smooth muscle cells in rTg-D rats. Brain sections from a WT rat (20 M) (A, C and E) or similarly aged rTg-D rat (22 M) (B, D and F) were immunolabeled with antibodies to collagen IV to identify cerebral blood vessels (red) or to smooth muscle cell α actin to identify smooth muscle cells (green) and stained with AmyGlo to visualize fibrillar amyloid (blue). Representative images from pial arteries (A and B); cortical arteries (C and D); and cortical arterioles (E and F). Scale bars = 50 µm.

Article Snippet: Staining for fibrillar amyloid was performed either using Amylo-Glo as described by the manufacturer (Biosensis Inc., Thebarton, South Australia) or staining with thioflavin S. The following antibodies were used for immunohistochemical analysis: rabbit polyclonal antibody to collagen type IV to visualize cerebral vessels (1:100; ThermoFisher, Rockford, IL); mouse monoclonal antibody to smooth muscle cell α actin (1:200, NBP 2-33006, Novus Bio); and antibody to HTRA1 (1:200, MAB2916, R&D Systems).

Techniques: Immunolabeling, Staining

Journal: Research

Article Title: Cell Mechanics Regulates the Dynamic Anisotropic Remodeling of Fibril Matrix at Large Scale

doi: 10.34133/research.0270

Figure Lengend Snippet: The role of integrin β1 and Piezo in fiber inductions. ASM cells were transfected with negative siRNA (100 nM) or double-positive siRNA for integrin β1 (ITGB1, 50 nM) and Piezo1 (50 nM). COL fiber inductions were recorded and quantified in geometrical parallelogram-arrayed clusters. (A to C) Fluorescent COL fibers and quantifications under the condition of cell clusters transfected with negative siRNA or double ITGB1 and Piezo1 siRNA. (D to F) Fluorescent COL fiber induction and quantification under the control condition (D), or with treatment of 5 μM Piezo inhibitor GsMTx4 (E) or 100 μM GdCl 3 (F). (G and H) Comparisons of the percentage changes in fiber growth (G) or cell spreading areas from the clusters (H) in control and Piezo inhibitor groups.

Article Snippet: Rat ASM cells were isolated from the tracheal tissue of 6- to 8-week-old female Sprague Dawley rats as described previously [ ].

Techniques: Transfection, Control